Technologies & Core Facilities
A key focus within the IGSP is the development of technologies to support the variety of research programs in the Institute, as well as across the Duke University and Medical Center campus. The IGSP, in collaboration with key partners, provides access to state-of-the-art genomic research tools, technologies, and expertise through a variety of core facilities, while at the same time engaging in the continual development and deployment of new technologies, providing the foundation for investigators to make important contributions to our understanding of complex biological and medical problems.
Gene Expression
The IGSP, with support from the Duke Comprehensive Cancer Center, has developed a range of technologies and services that enable genome-wide analysis of gene expression. The DNA Microarray Facility provides researchers with access to a suite of tools for the analysis of gene expression, including Affymetrix GeneChip arrays, custom spotted arrays, quantitative real-time PCR and Illumina BeadArray technologies as well as accompanying data analysis tools and expertise.
Genotyping
The IGSP, with support from the Duke Comprehensive Cancer Center, has developed an infrastructure to support research programs that focus on the analysis of genetic variation. The Genotyping Facility assists investigators in characterizing genetic variation, and offers tools and services to aid in the selection and analysis of SNPs by providing access to flexible, accurate, and affordable genotyping services.
RNA Interference
Recognizing the power of RNAi as a tool for facile genetic manipulation of cells and animals, the IGSP is developing a new facility to provide access to RNAi resources and services for the Duke faculty. The RNAi Facility serves as a focal point for RNAi expertise and resources on campus, and is devoted to developing and applying RNAi technologies for functional genomics studies.
DNA Sequencing
The IGSP offers a range of automated DNA sequencing options. Depending on project needs, researchers may access service levels ranging from limited-service DNA sequencing reaction clean-up and capillary sequencing, to large-scale project consultation, template preparation, sequencing, and downstream data handling.
Proteomics
The School of Medicine and the IGSP have collaboratively created a Proteomics Core Facility to provide protein characterization resources for the Duke Research Community. These services include protein identification and protein quantitation from a wide variety of sample types from simple mixtures (gel spots and bands) to complex mixtures (protein complexes, cell lysates, and plasma).
Biospecimen Repository
The IGSP is developing a standardized, regulatory-compliant and privacy protected Biospecimen Repository for the collection, processing, and banking of fluid and tissue biospecimens. This facility enables Duke investigators to reduce costs and eliminate significant redundancies and risks associated with alternative biobanking practices, while facilitating integration of clinical trials and translational research programs with molecular profiling technologies.