Pricing for Duke Scientists
Note - The Duke Comprehensive Cancer Center (DCCC) has generously supported the Proteomics Core Facility, therefore analyses performed for DCCC members working on peer reviewed, NCI funded projects receive a 5% discount. These researchers should contact Arthur Moseley (arthur.moseley@duke.edu) to receive this discount on their samples.
Sample Preparation
| Mini-Bradford Assay | $40 |
| In-Gel or In-solution digest | $40 |
| SPE Sample Cleanup (C18 or C8 Zip Tip) | $20 |
| MW Cutoff Sample Cleanup (Amicon 4) | $25 |
| Detergent/PEG Sample Cleanup (ProteoSpin) | $25 |
| IMAC or TiO2 purification for phosphopeptides | $40 |
| OFFGEL pI Fractionation (12 or 24 fraction, peptide or protein) | $750 |
| Single Stage Plasma depletion (Hu14 or Mouse3) | $300 |
| Two Stage Plasma Delpetion (IgY14 + Supermix) | $500 |
| Glycopeptide enrichment | $250 |
MW Determination
| Infusion or Fast LC/ESI/MS | $90 |
Qualitative Analyses
| LC-MS/MS analysis (gel spot/band - fast gradient) | $60 |
| LC-MS/MS analysis (moderately complex sample - med gradient) | $180 |
| LC-MS/MS analysis (complex sample -slow gradient) | $240 |
| 2D LC-MS/MS (per fraction) | $300 |
Quantitative Analyses
| LC-MSE Absolute Quantitation (single analysis) | $300 |
| LC-MSE Absolute Quantitation (triplicate analyses - technical replicates)) | $750 |
| Gel-free, Label-free Sample Differentiation (per sample, single analysis) | $300 |
| Gel-free, Label-free Differential Quantitation (per sample, triplicate analyses - technical replicates) | $750 |
| Absolute Quantitation Data Workup for Differential Expression | $150 |
| Isotope labeled expression analyses (iCAT/iTRAQ) | Contact |
| Targeted LC/MS/MS Quantitation Method Development | Contact |
| Targeted LC/MS/MS Quantitation (per sample - single analysis) | $300 |
| Targeted LC/MS/MS Quantitation (per sample - triplicate analyses - technical replicates) | $750 |
| mTRAQ Labeling for Targeted LC/MS/MS Quantitation (per sample) | $75 |
Data Analysis
| Custom Database Setup for MASCOT or Identity | $50 |
| Additional Database Searches | $20 |
| Consulting/Data analysis (per hour) | $65 |
Q1: I have a 1D gel band from a mouse cell line. How much will it cost to identify the proteins?
A1: For quick analysis (typically identifying between 1 and 10 proteins), we recommend using the fast gradient. For more complex samples, such as gel bands from IP pulldowns or molecular weight fractions of whole cell lysates, we recommend the medium gradient. The largest number of proteins reliably identified in our facility using a medium gradient from a gel band is 73 (approximately 400 peptides).
Fast Gradient:
| In-gel Digestion | $40 |
| LC/MS/MS analysis (gel band - medium gradient) | $60 |
| First pass database search (included) | $0 |
| Total Cost | $100 |
Medium Gradient:
| In-gel Digestion | $40 |
| LC/MS/MS analysis (gel band - medium gradient) | $180 |
| First pass database search (included) | $0 |
| Total Cost | $220 |
Q2: I have two immunoprecipitates, and I want to know what proteins differentiate the samples. How much will this cost?
A2: The researcher is looking for gross differences between these samples, thus this analysis does not require rigorous quantitation to differentiate the samples. A single LC - MSE run will be performed for each sample.
| Digestion (x2) | $80 |
| Gel-free, Label-free Sample Differentiation (test & control) | $600 |
| First pass database search (included) | $0 |
| Total Cost | $680 |
Q3: I am looking for subtle differences in protein expression in a cell line as a function of drug treatment. I am not sure what proteins will be affected. How much will this cost?
A3: To insure accurate quantitation by statistically accounting for analytical variability, it is recommended that each sample be analyzed in triplicate (technical replicates of LC/MS/MS).
| Bradford Assay and total protein normalization (x2) | $80 |
| Digestion (x2) | $80 |
| Gel-free, Label-free Sample Differentiation (test & control) | $1500 |
| First pass database search (included) | $0 |
| AbsQuant or Relative Quant data workup (x2) | $300 |
| Total Cost | $1960 |
To statistically account for biological and analytical variability, it is recommended that three biological replicates (each test and control) be analyzed, with three technical replicates of each biological replicate. This cost would be:
| Bradford Assay and total protein normalization (x2) | $240 |
| Digestion (x6) | $240 |
| Gel-free, Label-free Sample Differentiation (test & control) | $4500 |
| First pass database search (included) | $0 |
| AbsQuant or Relative Quant data workup (x6) | $1800 |
| Total Cost | $6780 |
Q4: I want to perform biomarker discovery in human plasma samples. How much will this cost per sample?
A4: It is recommended that 10% of the samples be analyzed in triplicate and 90% of the samples be analyzed in singlicate.
Singlicate Analysis - Single stage of plasma depletion (per sample)
| Bradford Assay and total protein normalization (x2) | $80 |
| Digestion | $40 |
| Depletion (Hu14) | $300 |
| Gel-free, Label-free Sample Differentiation | $300 |
| First pass database search (included) | $0 |
| AbsQuant or Relative Quant data workup | $150 |
| Total Cost | $870 |
Singlicate Analysis - Two stage plasma delpetion (per sample)
| Bradford Assay and total protein normalization (x2) | $80 |
| Digestion | $40 |
| Depletion (IgY14 + SuperMix)) | $500 |
| Gel-free, Label-free Sample Differentiation | $300 |
| First pass database search (included) | $0 |
| AbsQuant or Relative Quant data workup | $150 |
| Total Cost | $1,070 |
Triplicate Analysis - Single stage of plasma depletion (per sample)
| Bradford Assay and total protein normalization (x2) | $80 |
| Digestion | $40 |
| Depletion (Hu14) | $300 |
| Gel-free, Label-free Sample Differentiation | $750 |
| First pass database search (included) | $0 |
| AbsQuant or Relative Quant data workup | $150 |
| Total Cost | $1,320 |
Triplicate Analysis - Two stage plasma delpetion (per sample)
| Bradford Assay and total protein normalization (x2) | $80 |
| Digestion | $40 |
| Depletion (IgY + SuperMix)) | $500 |
| Gel-free, Label-free Sample Differentiation | $300 |
| First pass database search (included) | $0 |
| AbsQuant or Relative Quant data workup | $150 |
| Total Cost | $1,520 |
Q5: The species of interest to my study is not in the databases currently mounted. How can I appropriately process my data?
A5: We can upload your custom database to our Mascot or IdentityE server for database searching. This is a one-time fee, after which this database is available for your future use.
| Custom Database setup for MASCOT or IdentityE | $50 |