Duke Proteomics Core Facility

Protocols and Reagents

Protocols and Reagents

Sample Preparation Protocols

*SILAC reagents are provided to collaborators of the Core at significantly reduced cost.  Please contact us if you are interested.

Reference Peptides and Proteins

SpikeTides™ represent fully synthetic customized peptides, which terminate as native C-terminal heavily labeled or light Arg or Lys peptides or consist of a tryptic fragment that is fused to a proprietary JPT-tag. The latter tagged peptides enable a cost efficient access to quantified proteotypic peptides. JPT has developed a synthesis technology that enables ultra-fast, highly parallel and inexpensive synthesis of small scale peptides that are ideally suited for proteome wide profiling using SRM/MRM proteomic assays.
The sequences can be selected from bioinformatic prediction (i.e. Peptide Sieve) or from proteomic data repositories such as the Peptide Atlas (http://www.peptideatlas.org) that predict proteotypic peptides corresponding to peptides will be obtained from tryptic digestion of native proteomes.

OriGene Heavy-labeled Full-length Protein as MS Standards

OriGene now offers the service to generate Mass Spectrometry (MS) standards for 5,000 human proteins. Produced in human HEK293T cells and labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, the full length proteins with appropriate post-translational modifications are the best identification and quantification standards.

Superiority of OriGene’s MS standards:

  • Spiking at the early stage of sample process for accurate quantification.
  • Identify the best SRM and MRM transitions through experimental data.
  • Authentic posttranslational modifications by using human HEK293T cell line.
  • Higher data consistency than synthetic peptide internal standard.
  • Suitable for all types of MS equipments.
  • Over 90% incorporation efficiency.