Duke Proteomics Core Facility

Protocols and Reagents

Sample Preparation Protocols

Reference Peptides and Proteins

SpikeTides™ represent fully synthetic customized peptides, which terminate as native C-terminal heavily labeled or light Arg or Lys peptides or consist of a tryptic fragment that is fused to a proprietary JPT-tag. The latter tagged peptides enable a cost efficient access to quantified proteotypic peptides. JPT has developed a synthesis technology that enables ultra-fast, highly parallel and inexpensive synthesis of small scale peptides that are ideally suited for proteome wide profiling using SRM/MRM proteomic assays.
The sequences can be selected from bioinformatic prediction (i.e. Peptide Sieve) or from proteomic data repositories such as the Peptide Atlas (http://www.peptideatlas.org) that predict proteotypic peptides corresponding to peptides will be obtained from tryptic digestion of native proteomes.

OriGene Heavy-labeled Full-length Protein as MS Standards

OriGene now offers the service to generate Mass Spectrometry (MS) standards for 5,000 human proteins. Produced in human HEK293T cells and labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, the full length proteins with appropriate post-translational modifications are the best identification and quantification standards.

Superiority of OriGene’s MS standards:

Spiking at the early stage of sample process for accurate quantification.
Identify the best SRM and MRM transitions through experimental data.
Authentic posttranslational modifications by using human HEK293T cell line.
Higher data consistency than synthetic peptide internal standard.
Suitable for all types of MS equipments.
Over 90% incorporation efficiency.