shRNA Protocols
shRNA Plasmid Growth & Characterization (PDF file)
Protocols for bacterial growth, shRNA plasmid DNA preparation, characterization, and long-term storage of glycerol bacterial stocks.
Phoenix Retroviral Packaging:
Phoenix is a second-generation retrovirus producer lines for the generation of helper free ecotropic and amphotropic retroviruses generated by the Nolan Lab, and available from ATCC through the Duke Cell Culture Facility. Phoenix retroviral packaging protocols are available.
LinX Retroviral Packaging:
LinX retroviral producer cell line is available through OpenBiosystems. LinX retroviral packaging protocols (PDF file) are available.