A dynamic H3K27ac signature identifies VEGFA-stimulated endothelial enhancers and requires EP300 activity.
Zhang B, Day DS, Ho JW, Song L, Cao J, Christodoulou D, Seidman JG, Crawford GE, Park PJ, Pu WT, Genome Res. 2013 Apr 1;. Abstract
IGSP Faculty
Greg Crawford, PhDGreg Crawford, PhD
Assistant Professor, Department of Pediatrics, Division of Medical Genetics
My research involves identifying gene regulatory elements across the genome to help us understand how chromatin structure dictates cell function and fate.
Greg Crawford, Ph.D. is an Assistant Professor in the Department of Pediatrics and the IGSP. He received his Ph.D from the University of Michigan in 2001. Afterwards, he was a post-doctoral fellow in the lab of Dr. Francis Collins at the National Human Genome Research Institute, where he developed novel technologies to identify active gene regulatory elements on a genome-wide scale. He joined the Duke faculty in March 2006.
Research Statement
My research involves identifying gene regulatory elements across the genome to help us understand how chromatin structure dictates cell function and fate. For the last 25 years, mapping DNase I hypersensitive sites has been the gold standard method to identify the location of active regulatory elements, including promoters, enhancers, silencers, and locus control regions. I have developed two technologies that can identify most DNase I hypersensitive sites from potentially any cell type from any species with a sequenced genome. These data can be combined with other wet-lab and computational data types to better understand how these regulatory regions control global gene expression.
Learn more about Dr. Crawford's research in GenomeLIFE
Recent Publications
Patterns of regulatory activity across diverse human cell types predict tissue identity, transcription factor binding, and long-range interactions.
Sheffield NC, Thurman RE, Song L, Safi A, Stamatoyannopoulos JA, Lenhard B, Crawford GE, Furey TS, Genome Res. 2013 Apr 2;. Abstract
Early de novo DNA methylation and prolonged demethylation in the muscle lineage.
Tsumagari K, Baribault C, Terragni J, Varley KE, Gertz J, Pradhan S, Badoo M, Crain CM, Song L, Crawford GE, Myers RM, Lacey M, Ehrlich M, Epigenetics. 2013 Mar 1;8(3):317-32. Abstract
Synergistic and tunable human gene activation by combinations of synthetic transcription factors.
Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, Crawford GE, Hartemink AJ, Gersbach CA, Nat Methods. 2013 Mar;10(3):239-42. Abstract
Dynamic DNA methylation across diverse human cell lines and tissues.
Varley KE, Gertz J, Bowling KM, Parker SL, Reddy TE, Pauli-Behn F, Cross MK, Williams BA, Stamatoyannopoulos JA, Crawford GE, Absher DM, Wold BJ, Myers RM, Genome Res. 2013 Mar;23(3):555-67. Abstract
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